to differentiate between agarose gel electrophoresis and SDS-PAGE based on the following criteria:
2.2.1 Composition of gels
2.2.2 Dye used for staining

Answers

Answer 1

Explanation:

Agarose has a large pore size and is suitable for separating nucleic acids and large protein complexes. ... SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged.

Answer 2

In this question we will differentiate between agarose gel electrophoresis and SDS-PAGE based on the composition of gels and Dye used for staining.

Agarose gel

The gel matrix is ​​created by dissolving a natural polysaccharide called dye-free agarose, which is derived from a type of seaweed, in a conductive buffer, in the approximate proportion of 1% to 3% agarose.

SDS-PAGE

Polyacrylamide gel electrophoresis (PAGE) with sodium dodecyl sulfate (SDS). SDS-PAGE consists of a type of electrophoresis performed under denaturing conditions for the separation of proteins without dyes.

Whit this information, we can conclude that differentiate between agarose gel electrophoresis and SDS-PAGE based on the composition of gels and Dye used for staining.

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Related Questions

The process by which natural variation among organisms that make them best adapted to their environment live and reproduce, therefore passing on to their offspring the genetic qualities best suited to that environment
variation
variation

adaptation
adaptation

natural selection
natural selection

Evolution

Answers

Answer:

Natural Selection

Anoles are a family of IIzards found throughout the Americas. Green anoles, Anolis carolinensis, are native to i specific area of Florida. In this
area, the primary territory of green anoles was once along the ground and in low-level branches. However, the Introduction of brown anoles,
Anolis sagrei, from Cuba forced green anoles to shift o their territory to the tops of trees. The branches at the treetops are smoother and thinner
than those close to the ground. Over time, the toe pads of the green anoles have become larger and stickler.
What is the best explanation for the change In green anoles' toe pads?
Some individuals In the green anole population had mutations that helped them survive and reproduce In the new conditions,
so thelr genes were passed on to the next generation.
O B. Gene flow between the green and brown anoles provided the green anoles with traits that helped them survive and
reproduce, and those tralts were passed on to the next generation.
The feet of the green anoles became larger and stickler as they used them, which helped the green anoles to better survive,
reproduce, and pass the tralt on to the next generation.
O D. The change in conditions caused mutations In green anoles that gave them beneficial tralts for survival and reproduction, and
those mutations were passed on to the next generation.
Reset
Next
dmentum. All rights reserved.
ED INTL

Answers

Answer: D

Explanation:

Answer:

Some individuals in the green anole population had mutations that helped them survive and reproduce in the new conditions, so their genes were passed on to the next generation.

Explanation:

what is process of forming an exact copy of the dna molecule called?

Answers

it’s:
dna replication

Helppppp Plzzzzzzz!!! 15+PTS and brainliest!!!!!

What upward force is produced by the asthenosphere?

Select one of the following.

A: Buoyant Force
B: Gravitational Force

Answers

Answer:

A: Buoyant Force

[tex] \huge \frak \green{question}[/tex]

What upward force is produced by the asthenosphere?

[tex] \huge \frak \pink{answer}[/tex]

Buoyant Force

How many mL of stock solution of 2M NaCl do you need to prepare 100 mL of 0.150M NaCl?​

Answers

Answer:

7.5mL

Explanation:

Comsidering the definition of dilution, 7.5 mL of the stock solution  of 2 M are required to prepare 100 mL of 0.150 M solution.

Dilution is a procedure by which the concentration of a solution is lowered. It is achieved by adding more solvent to the same amount of solute.

Then the amount of solute does not vary, but the volume of the solvent does: when more solvent is added, the concentration of the solute decreases, as the volume of the solution increases.

A dilution is mathematically expressed as:

Ci×Vi = Cf×Vf

where

Ci: initial concentration Vi: initial volume Cf: final concentration Vf: final volume

In this case, you know:

Ci= 2 M  Vi= ? Cf= 0.150 M Vf= 100 mL  

Replacing in the definition of dilution:  

2 M×Vi= 0.150 M×100 mL

Solving:

[tex]Vi=\frac{0.150 M x 100 mL}{2 M}[/tex]

Vi= 7.5 mL

In summary, 7.5 mL of the stock solution  of 2 M are required to prepare 100 mL of 0.150 M solution.

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This category of endangerment has so few individual survivors that the species could soon become extinct:

A. Invasive species
B. Threatened species
C. Endangered species
D. Endemic species
E. Red list species

Answers

Answer:

Endangered Species

Explanation:

C. Endangered species

How many tropic levels are there in the following food chain?

algae —> mosquito larvae —> dragonfly larvae —> trout

Answers

Answer:

There are 4 trophic levels in the following food chain.

Which statement describes a non-native species?

A. It is a poor predator.

B. It increases stability of a community.

C. It has been introduced to a new area.

D. It has no permanent home.

Answers

Answer:

c

Explanation:

a non-native species is a species that isn't familiar/native to the place. if it is introduced or moved to a new area, then it is non-native.

Which statement provides the best argument against making major changes to the atmosphere to help protect Earth’s resources? There will be less soil and dust in the air as construction increases. The atmosphere will improve as more people move to large cities. Making major changes to the atmosphere can be a costly process. It is more important to protect land and water resources than the atmosphere.

Answers

The answer is C. Making major changes to the atmosphere can be a costly process

Explanation:

Making major changes to the atmosphere can be a costly process.

The atmosphere refers to the layer of thin gases that lie above the surface of the earth. Plants and animals depend on the atmosphere for survival because it is region in which gaseous exchange takes place.

Changes to the atmosphere is very costly because it involves changes such as alteration in the kind of cars that are used and devising of alternative sources of energy etc. All these are expensive adventures.

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Describe the flow of a river from beginning to the end


Use the following terms: headwaters, springs, tributaries, confluence, and mouth

Answers

The flow of a river from the beginning is called the headwater, as its where the water is coming from. Springs are warmer water spots in a big or little space of water. Confluence is when two rivers connect into one. Tributaries are caused when rivers flow out into a larger river or lake. The mouth of the river flows from a larger source into a smaller one, commonly a stream or river.

The flow of river begins with the Headwater .

Springs are warmer water bodies compared to rivers,

The rivers connect to another river to form a  confluence  

Rivers flow into larger water bodies in the form of tributaries.

The mouth of the river flows from a larger source to a smaller body of water.

Describing the flow of a river

The flow of river begins with the Headwater ( the beginning of the river ), springs are warmer water bodies compared to rivers, When the rivers flow they connect to another river ( confluence ) which flow into larger rivers in the form of tributaries while the mouth of the river flows form a larger source to a smaller body of water.

Hence we can conclude that the flow of river is as described above

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One scientist kept 20 grams of a radioactive material in a container. What will be the
mass of the radioactive material left in the container after 2 half lives?

40 grams
4 grams
10 grams
5 grams

Answers

After one half-life, there would be 10 g of the material left. After two half-lives, there will be 5 g of the material left.

After one half-life, there would be 10 g of the material left.

After two half-lives, there will be 5 g of the material left.

Therefore, 5 grams is the correct answer.

What is the unit of grams?

The basic unit of mass or weight in the metric system is the gram. The most frequently used units of mass or weight used in the Canadian food industry are the gram and kilogram. Some of the types of grams are:-

Unit           Abbreviation Mass (Weight)

kilogram  kg                 1000 grams

hectogram hg                 100 grams

decagram dag                  10 grams

gram         g                   1 gram

Therefore, last option is the correct answer.

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AG CLASS

Which of the following professionals is most likely to be hired to mow the grass and lawns of an urban park and perform common maintenance chores?


landscape architect

landscape contractor

landscape worker

landscape designer

Answers

the answer is landscape worker!

Why do you need to breathe in oxygen?
a.Oxygen is needed to split glucose.
b.Oxygen provides energy that is to be put into ATP.
c.Oxygen pulls electrons through the process of cell respiration.
d.Oxygen reduces the NAD+ and FADH to produce NADH and FADH2

Answers

C. O2 pulls electrons through the process of cell respiration

Answer:

a

Explanation:

Breathing in of oxygen is respiration. And respiration is the taken in of oxygen to break down glucose and energy in form of ATP as by product

Please select the word from the list that best fits the definition

Grouping items together to keep observations organized


inferring


predicting


classifying


observing

Answers

Answer:

classifying

Explanation:

for example like classifying the files from SVU classifying them keeps them more organized and labeled kind of like identifying them classifying them keeps them all together in one group

8. Which is true about viruses!
a. They are prokaryotic cells that infect living organisms
b. They are nonliving particles that infect living organisms
C. They are eukaryotic cells that infect living organisms
d. They are fungi that infect living organisms

Answers

Answer:

The Answer is gonna be A. They are prokaryotic cells that infect living organisms

This is the right Answer :3

I hope you are having a great day ❤️❤️❤️❤️

the answer is A a virus is a prokaryotic cell

Which of the following statements are true about the esophagus? Select all that apply.
The esophagus is a bony tube.
The esophagus is a muscular tube.
The esophagus moves food from the mouth to the small intestine.
The esophagus moves food from the mouth to the stomach.
Which of the following statements are true about the esophagus?

Answers

The statements that are true about the esophagus are:

The esophagus is a muscular tube.

The esophagus is the muscular tube of the digestive tract that develops from the pharynx to the stomach.

The esophagus moves food from the mouth to the stomach.

This part of the digestive system is traversed by food, which enters through the mouth, passes through the pharynx, crosses the esophagus and reaches the stomach.

In this process, the esophagus has an active participation, since its walls contract to promote the advance of the food.

Therefore, we can conclude that the esophagus is a muscular tube that moves food from the mouth to the stomach.

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Plants have many characteristics.

What is responsible for these characteristics?

Select all that apply.


inherited from parents

inherited by sibling plants

affected by the environment

affected by soil position

Answers

Answer:bnunununun Noicd

Explanation: skssssss

1. Which change is an environmental effect of building dams?

A) weed growth upstream

B) increased fertility downstream

C) increased water temperature upstream

D) sediment builds up downstream


2. How can humans increase the rate of endangered or extinct species?

A) reduce environmental impacts

B) build and populate more zoos

C) pass more laws about land use

D) introduce new predators to an environment

Answers

Answer: 1. C) increased water temperature upstream. 2. D) introduce new predators to an environment. brainliest??

Explanation:

The change that is an environmental effect of building dams is increased water temperature upstream, which is in Option C, and humans can increase the rate of endangered or extinct species by reducing environmental impacts, which is in Option A.

What is Human activities on species?

Human activities are the primary cause of species endangerment and extinction, so humans must take actions to reduce environmental impacts in order to increase the rate of endangered or extinct species, and by reducing hunting and poaching and restoring degraded ecosystems can help protect endangered species, and reducing carbon emissions and promoting sustainable development can help mitigate the effects of climate change.

Hence, the effect of building dams is increased water temperature upstream, which is Option C, and humans can increase the rate of endangered or extinct species by reducing environmental impacts, which is Option A.

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what is the outcome of cell signaling

Answers

Answer:

Other important large-scale outcomes of cell signaling include cell migration, changes in cell identity, and induction of apoptosis (programmed cell death).

Explanation:

What is the genetic make-up of an organism?

Answers

Answer:

Explanation:

In a broad sense,genetic makeup of an organism can be regarded as the

"genotype" . It gives description about

complete set of genes an organism.

If we narrow it, it can be reffered to as "alleles" , this is is variant forms of a gene which is been carried by an organism. For instance, Humans can be regarded as diploid organisms, i.e there are two alleles at their each genetic position along with one allele which is been inherited from each parent. genotype of a specific gene is been represented by "Each pair of alleles". Instance of this is of "sweet pea plants" the gene responsible for flowering color posses two alleles, One of the alleles is responsible for coding purple flowers, symbolized with

uppercase letter F, the other allele is responsible for coding of white flowers

symbolized with Lowercase letter" f "

There are three possible genotypes which could be featured for Different population of a sweet pea, which are

✓FF

✓Ff

✓ ff.

Genotype of each plant have some contribution to the phenotype, by saying this, we mean outward appearance of its flowers.

Describe the differences and similarities between lecithin and cephalin. Match the words in the left column to the appropriate blanks in the sentences on the right. ResetHelp Lecithins and cephalins are both sphingolipids; they consist of glycerol with fatty acids forming bonds with the first and second groups. The third group of the forms a with phosphoric acid, which forms another bond with an amino alcohol. In lecithins, the amino alcohol is ; in cephalins, the amino alcohol is usually .

Answers

Answer:

Here, we have a composition passage and we are required to fill in the with missing words. The missing words are highlighted in bold format and underlined.

Lecithins and cephalins are both glycerophospholipids; they consist of glycerol with fatty acids forming ester bonds with the first and second hydroxyl groups. The third hydroxyl group of the glycerol forms a phosphate bond with phosphoric acid, which forms another phosphate bond with an amino alcohol. In lecithins, the amino alcohol is choline; in cephalins, the amino alcohol is usually ethanolamine.

Explanation:

Lecithin is a chemical complex composed primarily of fatty acids, glycerol, phosphoric acid, and choline. It can be present in egg yolks, soybeans, sunflower seeds, and plant seed cells and is completely normal. Soybean and sunflower lecithins contain much more important fatty acids than rapeseed lecithin, making them nutritionally more useful.

Phosphatidylethanolamine, also known as cephalin, is a phosphoglyceride that, like phosphatidylcholines and lecithins, is a component of biological membranes. A glycerol residue esterified by two fatty acids and a phosphoethanolamine residue gives rise to phosphatidylethanolamines. They're made by combining CDP-ethanolamine with diglycerides and releasing a CMP molecule. Phosphatidylcholines are formed when S-adenosylmethionine methylates the amine in ethanolamines. They're mostly found in the inner layer of plasma membranes' lipid bilayers.

The more diverse an ecosystem is-
A- the more threatened it’s populations are
B- the more similar the species will be
C- the more sustainable it is
D- the fewer varieties of species are present

Answers

Answer:b

Explanation:

Which of the following measures would improve energy
efficiency/conserve energy?
1. Increasing fuel economy standards on cars
2. Purchasing larger in-home water heaters
3. Unpluging electronics when not in use
4. Eating more locally sourced food
5. Removing tax incentives on home-solar purchases
O i, ii, iii, iv, and v
O i and iv only
O i, iii, and iv
O i, ii, and iii

Answers

Answer:

l

Explanation:

i’ll give brainliest :) !!

Root hairs, a part of the root tissue system, does what?

Answers

Answer:

The third option is correct.

Hope this helps.

During the rock cycle, ______________ follows erosion. *

Answers

Answer:

deposition

Explanation:

Hope this helps and have a great day!!!!

[tex]\small\sf{During\: the\: rock \:cycle, \:{\underline{\bf{\red{\:\:\:\:Deposition\:\:\:\:}}}}follows\: erosion.}[/tex]

Jaguars are a keystone species in the Amazon. Describe how they can be so essential to the ecosystem despite
being significantly less abundant than many other species.

Answers

Answer:

jaguars are keystone species because they feed herbivorous species that might decimate the plants of the ecosystem

Explanation:

Keynote species are species that help to maintain the homeostasis of the ecosystem so if they lack then the ecosystem would change drastically. Moreover, a top predator is a predator that occupies the highest trophic level in the food chain. Jaguars are both keystone species and top predators that feed herbivorous species, maintaining the health of the food chain by eliminating the excess of primary consumers that otherwise would feed too many plants (i.e., primary producers) which produce and store energy at the bottom of the food chain.

Which statements describe the effects of indoor pollution? Select two options.
Ultrafine particles cause poor health.
Nose hairs and mucus catch all the particles before they reach the lungs
Ultrafine particles are absorbed by the lungs when people breathe.
Ultrafine particles contribute to more than 20,000 deaths per year in the United States.
Most ultrafine particles can be seen with the unaided eye.

Answers

Answer:

A and C

Explanation:

The negative health impacts of indoor pollution are caused by ultrafine particles that are inhaled and absorbed by the lungs. So, the correct options are A and C.

What are Indoor pollution?

Indoor air pollution, which can be as much as ten times worse than outside air pollution, is indeed the degradation in indoor air quality caused by hazardous chemicals and other elements. This is because enclosed rooms encourage the accumulation of possible contaminants more than do open ones.

The main contributors to indoor air pollution include: Bioaerosols, building materials, and combustion Although radon, asbestos, pesticides, toxic substances, organic volatile compounds, and environmental tobacco smoke are all regarded as serious indoor pollutants in industrialised countries, developing countries are primarily affected by the combustion byproducts of biomass fuels.

The negative health impacts of indoor pollution are caused by ultrafine particles that are inhaled and absorbed by the lungs.

Therefore, the correct options are A and C.

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Carbon is found in the
atmosphere in which form?

A. calcium carbonate
B. carbon dioxide
C. carbonic acid

Answers

CARBON IS FOUND IN YHE ATMOSPHERE IN THE FORM OF carbon dioxide or CO2

Which of the following scenarios will decrease the genetic variation of a population?

Answers

Answer:

population of birds suddenly decrease their frequency of  sexual reproduction.

Explanation:

This is the answer to quiz accordingly.

(blank) measures how much space matter takes up.

Answers

Answer:

volume

Explanation:

it's volume I think

Other Questions
9/166 in fraction from? 50 POINTS Vanessa has 6 dimes and 2 pennies. Joachim has 1 dollar, 3 dimes, and 5 pennies. Jimmy has 5 dollars and 7 pennies. They want to put their money together to buy a game that costs $8.00. Do they have enough money to buy the game? If not, how much more money do they need? SHOW WORK 1 kilogram 900 is it more than less than or equal to plz help plzzzz help meeeeeeeeeeee VIII Que font-ils? Tell what various people are doing by completing the sentences with the correct form of the most logical verb in Present tense. Each verb is used ONCE ONLY: aimer, arrive(M), avoir, tre commencer ,assister, partager, regarder, quitter, dner, retrouver, travailler (10 pts.)Modle: Elles ______________ toujours lheure. The most logical verb(arriver/conjugated arrivent 1. Matilde_________________________________ aux cours a la fac. 2. Mes parents _________________________________ ensemble luniversit. 3. Vous ____________________________ au restaurant U avec vos copains.4. Mon colocataire et moi, nous______________________________ un petit appartement. 5. Elles ________________belles. Elles___________ les cheveux blonds et les yeux bleus.6. Mon premier cours __________________ 9h30, alors je _____________la maison 8h45. 7. Le week-end, mes amie et moi________________________________ un film au cinma. 8. Sarah et La ______________________________ rendre visite leurs parents le week-end. . after the Mexican American War what new state applied for Statehood? and what year? And was this state a Free State or Slave State? Please help 30 points The volume of this rectangular prism is 53.72 cubic centimeters . what is the value of y? Suppose you are given the function f(x) = 7x and asked for f(-6). This is the same as asking for the value of the function at x = ? Damian is trying to pick out an outfit for the first day of school. He can choose from 3 pairs of pants, 5 t-shirts, 8 sweaters or hoodies, and 5 pairs of shoes. How many different outfits does Damian have to choose from? (Don't answer if you don't know and no bots with the fake links pls) Marie bicycled 15 miles at 5 miles perhour. How long did Marie bicycle? What is needed for evolution to take place? Q3: State whether each of the following is true or false. If false, explain why. 1. A generic method cannot have the same method name as a nongeneric method. 2. All generic method declarations have a type-parameter section that immediately precedesthe method name. 3. A generic method can be overloaded by another generic method with the same methodname but different method parameters. 4. A type parameter can be declared only once in the type-parameter section but can appearmore than once in the methods parameter list. 5. Type-parameter names among different generic methods must be unique. 6. The scope of a generic classs type parameter is the entire class except its staticmembers. natural selection vs artificial whats the difference Will give BRAINLIEST How are animalsaffected by succession? explain whether or not there are absolute answers to moral questions (such as "what does it mean to be a good person"). In other words, if I think it is always wrong to lie, and you think lying is alright to spare another's feelings, are you wrong or am I wrong or are we both right for ourselves? In goodness relative or absolute? need help guys givin brainliest A circle has a radius of 8 centimeters. The measure of the arc of theshaded region is 72. Which is closest to the area of the shaded section wright this without exponents -2u^5*6u^3 find the volume................